Simultaneous estimation of xanthine alkaloids (Theophylline, Theobromine and Caffeine) by High-Performance Liquid Chromatography

  • Shruti Mourya
  • Ramesh Bodla
  • Ravikant Taurean
  • Akanksha Sharma
DOI https://doi.org/10.22270/ijdra.v7i2.315

Abstract

Methylxanthines are mainly a group of phytochemicals which are derived from purine base xanthine. These xanthines are obtained from plant as a result of secondary metabolism. There are various physiological actions that have been attributed to these derivatives in neurogenerative diseases, respiratory diseases, cancer and diabetes. The aim of this study is to develop a suitable qualitative and quantitative method for these xanthine derivatives. HPLC method is suitable for simultaneous estimation of methylxanthines, based on their physicochemical properties. Theobromine, Theophylline and Caffeine that belongs to alkaloids possess their economic effects. They have various stimulant effects on cardiovascular system, gastrointestinal system, respiratory system, central nervous system etc. that results in increased motivation to work, increased energy and increased alertness. High performance liquid chromatography is used for simultaneous determination of theophylline, theobromine and caffeine from different tea leaves.

Keywords: Xanthine alkaloids, Theobromine, Theophylline, Caffeine, Xanthines

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1.
Mourya S, Bodla R, Taurean R, Sharma A. Simultaneous estimation of xanthine alkaloids (Theophylline, Theobromine and Caffeine) by High-Performance Liquid Chromatography. Int J Drug Reg Affairs [Internet]. 2019Jun.16 [cited 2024Dec.8];7(2):35-1. Available from: https://www.ijdra.com/index.php/journal/article/view/315
Issue
Vol 7 No 2 (2019): VOLUME 7, ISSUE 2, 2019
Section
Review
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